Filtering is performed using BBTools rqcfilter.sh script.

Fastq Files

Number of Reads File Size File Location
Input FASTQ [_RAW-READ-CNT_] [_RAW-FILE-SIZE_] MB [_RAW-FILE-LOCATION_]
Filtered FASTQ [_FILTERED-READ-CNT_] [_FILTERED-FILE-SIZE_] MB [_FILTERED-FILE-LOCATION_]
Filtered % [_REMOVED-READ-PCT_]% - -
The file size for the filtered fastq is significantly smaller than the input fastq because the reads were re-ordered to optimize gzip file compression.

Filtering Metrics

Metric Read Count Base Count
Input [_RAW-READ-CNT_] [_RAW-BASE-CNT_]
Remaining [_FILTERED-READ-CNT_] [_FILTERED-BASE-CNT_]
Percentage Removed [_REMOVED-READ-PCT_]% [_REMOVED-BASE-PCT_]%

Metric Percent Read Count
Low Quality Removed [_LOW-QUAL-REMOVED-PCT_] [_LOW-QUAL-REMOVED-READ-CNT_]
Artifact Removed [_ARTI-REMOVED-PCT_] [_ARTI-REMOVED-READ-CNT_]
Ribosomal RNA Removed [_RRNA-REMOVED-READ-PCT_] [_RRNA-REMOVED-READ-CNT_]
Microbial Removed [_MICROBE-REMOVED-READ-PCT_] [_MICROBE-REMOVED-READ-CNT_]
Human Filtered [_HUMAN-REMOVED-READ-PCT_] [_HUMAN-REMOVED-READ-CNT_]
Dog Filtered [_DOG-REMOVED-READ-PCT_] [_DOG-REMOVED-READ-CNT_]
Cat Filtered [_CAT-REMOVED-READ-PCT_] [_CAT-REMOVED-READ-CNT_]
Mouse Filtered [_MOUSE-REMOVED-READ-PCT_] [_MOUSE-REMOVED-READ-CNT_]

[_FILTERED-READ-QC_]